Registro:
Título en inglés:
Genetic dissection of the degradation pathways for the mycotoxin fusaric acid in Burkholderia ambifaria T16
Autor/es:
Vinacour, Matías; Moiana, Mauro; Forné, Ignasi; Jung, Kirsten; Bertea, Micaela; Calero Valdayo, Patricia M. ; Nikel, Pablo I.; Imhof, Axel; Palumbo, Miranda C.; Fernández Do Porto, Darío Augusto; Ruiz, Jimena A.
Filiación:
Vinacour, Matías. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Vinacour, Matías. CONICET – Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Moiana, Mauro. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Moiana, Mauro. CONICET – Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Forné, Ignasi. Ludwig - Maximilians - Universität. Protein Analysis Unit, BioMedical Center (BMC). München, Martinsried, Germany.
Jung, Kirsten. Ludwig - Maximilians - Universität. Faculty Biology, Microbiology. München, Martinsried, Germany.
Bertea, Micaela. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Bertea, Micaela. CONICET – Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Calero Valdayo, Patricia M. Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability. Kongens Lyngby, Denmark.
Nikel, Pablo I. Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability. Kongens Lyngby, Denmark.
Imhof, Axel. Ludwig - Maximilians - Universität. Protein Analysis Unit, BioMedical Center (BMC). München, Martinsried, Germany.
Palumbo, Miranda C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo (IC). Buenos Aires, Argentina.
Palumbo, Miranda C. CONICET - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo (IC). Buenos Aires, Argentina.
Fernández Do Porto, Darío Augusto. Universidad de Buenos Aires. Instituto de Cálculo (IC). Buenos Aires, Argentina.
Fernández Do Porto, Darío Augusto. CONICET - Universidad de Buenos Aires. Instituto de Cálculo (IC). Buenos Aires, Argentina.
Fernández Do Porto, Darío Augusto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires, Argentina.
Ruiz, Jimena A. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Ruiz, Jimena A. CONICET – Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Ruiz, Jimena A. Ludwig - Maximilians - Universität. Faculty Biology, Microbiology. München, Martinsried, Germany.
Ruiz, Jimena A. Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability. Kongens Lyngby, Denmark.
Título revista:
Applied and Environmental Microbiology
Temas:
BURKHOLDERIA AMBIFARIA T16; FUSARIC ACID; TWO - COMPONENT FLAVIN - DEPENDENT MONOOXYGENASE; 2 - METHYLCITRATE CYCLE; DETOXIFICATION; CATABOLISM
Url al documento en Intranet FAUBA:
Resumen:
Fusaric acid (FA) is a mycotoxin produced by several Fusarium species. Burkholderia ambifaria T16 is a rhizosphere bacterium, able to use FA as sole nitrogen, carbon, and energy source. By screening a transposon insertional library, combined with proteomic analysis, genes and enzymes involved in the microbial degradation of FA were identified for the first time. A functional 2-methylcitrate cycle, an anaplerotic pathway where propionyl-coenzyme A (CoA) is converted to pyruvate and succinate, was shown to be essential for growth in the presence of FA. The proteomic profile of B. ambifaria T16 showed that more than 50 enzymes (including those belonging to the 2-methylcitrate cycle, fatty acid metabolism, valine catabolism, and flavin biosynthesis) were significantly more abundant when growing on FA than on citrate. Flavin mononucleotide (FMN)- dependent luciferases like monooxygenase (LLM) are shown to catalyze the pyridine-ring cleavage reaction of several N-heterocyclic compounds. Deletion of a gene encoding a predicted LLM enzyme that was highly upregulated during growth on FA, completely abolished the capability of B. ambifaria T16 to grow with this mycotoxin as sole nitrogen, carbon, and energy source. Re-introduction of the wild type gene was able to restore growth. The mentioned gene is part of a gene cluster of unknown function that we termed fua, due to its probable role in fusaric acid catabolism. Our results suggest that the LLM encoded in the fua cluster catalyzes the pyridine-ring opening reaction during FA degradation, and that propionyl-CoA is one of the intermediates of FA catabolism in B. ambifaria T16.
Citación:
---------- APA ----------
Vinacour, M.; Moiana, M.; Forné, I.; Jung, K.; Bertea, M.; Calero Valdayo, P. M. ; Nikel, P. I.; Imhof, A.; Palumbo, M. C.; Fernández Do Porto, D. A. & Ruiz, J. A. (2023).Genetic dissection of the degradation pathways for the mycotoxin fusaric acid in Burkholderia ambifaria T16.Applied and Environmental Microbiology,89, (12),art.3
10.1128/aem.00630-23
---------- CHICAGO ----------
Vinacour, Matías,Moiana, Mauro,Forné, Ignasi,Jung, Kirsten,Bertea, Micaela,Calero Valdayo, Patricia M. , et al..2023. "Genetic dissection of the degradation pathways for the mycotoxin fusaric acid in Burkholderia ambifaria T16".Applied and Environmental Microbiology 89, no.12:art.3.
Recuperado de http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2024vinacour