Registro:
Título en inglés:
Preimplantation developmental competence of bovine and porcine oocytes activated by zinc chelation
Autor/es:
Cabeza, Juan Pedro; Camera, Juan; Briski, Olinda; Yauri Felipe, Minerva; Salamone, Daniel Felipe; Gambini, Andrés
Filiación:
Cabeza, Juan Pedro. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Camera, Juan. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Briski, Olinda. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Briski, Olinda. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina.
Briski, Olinda. CONICET - Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina.
Yauri Felipe, Minerva. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Yauri Felipe, Minerva. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina.
Yauri Felipe, Minerva. CONICET - Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina.
Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina.
Salamone, Daniel Felipe. CONICET - Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigaciones en Producción Animal (INPA). Buenos Aires, Argentina.
Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Gambini, Andrés. The University of Queensland. School of Agriculture and Food Sciences. Gatton, Australia.
Temas:
ASSISTED OOCYTE ACTIVATION; EMBRYO DEVELOPMENT; OOCYTE TO EMBRYO TRANSITION; ZINC CHELATOR; CALCIUM OSCILLATION
Resumen:
After sperm - oocyte fusion, intracytoplasmic rises of calcium (Ca) induce the release of zinc (Zn) out of the oocyte (Zn sparks). Both phenomena are known to play an essential role in the oocyte activation process. Our work aimed to explore different protocols for activating bovine and porcine oocytes using the novel zinc chelator 1,10 - phenanthroline (PHEN) and to compare developmental rates and quality to bovine IVF and parthenogenetic ionomycin - induced embryos in both species. Different incubation conditions for the zinc chelator were tested, including its combination with ionomycin. Embryo quality was assessed by immunofluorescence of SOX2, SOX17, OCT4, and CDX2 and total cell number at the blastocyst stage. Even though blastocyst development was achieved using a zinc chelator in bovine, bypassing calcium oscillations, developmental rates, and blastocyst quality were compromised compared to embryos generated with sperm - induced or ionomycin calcium rise. On the contrary, zinc chelation is sufficient to trigger oocyte activation in porcine. Additionally, we determined the optimal exposure to PHEN for this species. Zinc chelation and artificial induction of calcium rise combined did not improve developmental competence. Our results contribute to understanding the role of zinc during oocyte activation and preimplantation embryo development across different mammalian species.
Citación:
---------- APA ----------
Cabeza, J. P.; Camera, J.; Briski, O.; Yauri Felipe, M.; Salamone, D. F. & Gambini, A. (2022).Preimplantation developmental competence of bovine and porcine oocytes activated by zinc chelation.Animals,12, (24),art.3560
10.3390/ani12243560
---------- CHICAGO ----------
Cabeza, Juan Pedro, Camera, Juan, Briski, Olinda, Yauri Felipe, Minerva, Salamone, Daniel Felipe, Gambini, Andrés.2022. "Preimplantation developmental competence of bovine and porcine oocytes activated by zinc chelation".Animals 12, no.24:art.3560.
Recuperado de http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2022cabeza