Registro:
Título en inglés:
Characterization of a novel GH10 alkali - thermostable xylanase from a termite microbiome
Autor/es:
Mon, María Laura; Marrero Díaz de Villegas, Rubén; Campos, Eleonora; Soria, Marcelo Abel; Talía, Paola M.
Filiación:
Mon, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
Mon, María Laura. CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
Marrero Díaz de Villegas, Rubén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
Marrero Díaz de Villegas, Rubén. CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
Campos, Eleonora. CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Soria, Marcelo Abel. CONICET – Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Talía, Paola M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
Talía, Paola M. CONICET - Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
Título revista:
Bioresources and Bioprocessing
Temas:
GH10; ENDOXYLANASE; BIOCHEMICAL CHARACTERIZATION; TERMITE GUT MICROBIOME
Resumen:
The aim of the present study was to assess the biochemical and molecular structural characteristics of a novel alkalithermostable GH10 xylanase (Xyl10B) identified in a termite gut microbiome by a shotgun metagenomic approach. This endoxylanase candidate was amplified, cloned, heterologously expressed in Escherichia coli and purified. The recombinant enzyme was active at a broad range of temperatures (37 – 60 ºC) and pH values (4 – 10), with optimal activity at 50 ºC and pH 9. Moreover, its activity remained at more than 80% of its maximum at 50 °C for 8 h. In addition, Xyl10B was found to be stable in the presence of salt and several ions and chemical reagents frequently used in the industry. These characteristics make this enzyme an interesting candidate for pulp and paper bleaching industries, since this process requires enzymes without cellulase activity and resistant to high temperatures and alkaline pH (thermo - alkaliphilic enzymes). The products of xylan hydrolysis by Xyl10B (short xylooligosaccharides, xylose and xylobiose) could be suitable for application as prebiotics and in the production of bioethanol. Key points: 1. Biochemical and molecular structural characterization of a novel GH10 xylanase (Xyl10B) from a termite gut microbiome, 2. Xyl10B is a candidate biocatalyst in the bleaching process of pulp and the paper industries because of its inactivity on carboxymethyl cellulose, 3. The shorter xylooligosaccharides generated from the hydrolysis of xylan would be suitable in different applications, including the food industry as prebiotics.
Citación:
---------- APA ----------
Mon, M. L.; Marrero Díaz de Villegas, R.; Campos, E.; Soria, M. A. & Talía, P. M. (2022).Characterization of a novel GH10 alkali - thermostable xylanase from a termite microbiome.Bioresources and Bioprocessing,9, (1),art.84
10.1186/s40643-022-00572-w
---------- CHICAGO ----------
Mon, María Laura, Marrero Díaz de Villegas, Rubén, Campos, Eleonora, Soria, Marcelo Abel, Talía, Paola M..2022. "Characterization of a novel GH10 alkali - thermostable xylanase from a termite microbiome".Bioresources and Bioprocessing 9, no.1:art.84.
Recuperado de http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2022mon