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Conde Molina, D.; Novelli Poisson, G. F.; Kronberg, M. F.; & Galvagno, M. A. (2021)"Valorization of an Andean crop (yacon) through the production of a yeast cell - bound phytase". Biocatalysis and Agricultural Biotecnology,36,e102116, 8 p.

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Documento:
Artículo
Título en inglés:
Valorization of an Andean crop (yacon) through the production of a yeast cell - bound phytase
Autor/es:
Conde Molina, Débora; Novelli Poisson, Guido Fernando; Kronberg, María Florencia; Galvagno, Miguel Angel
Filiación:
Conde Molina, Débora. Universidad Tecnológica Nacional. Facultad Regional Delta. Departamento de Ingeniería Química. Laboratorio de Biotecnología. Campana, Buenos Aires, Argentina.
Novelli Poisson, Guido Fernando. Universidad de Buenos Aires. Facultad de Ingeniería. Departamento de Ingeniería Química. Laboratorio de Microbiología Industrial. Ciudad Universitaria, Buenos Aires, Argentina.
Novelli Poisson, Guido Fernando. Universidad Nacional de San Martín (UNSAM). Instituto de Investigaciones Biotecnológicas (IIBIO). San Martín, Buenos Aires, Argentina.
Novelli Poisson, Guido Fernando. CONICET - Universidad Nacional de San Martín (UNSAM). Instituto de Investigaciones Biotecnológicas (IIBIO). San Martín, Buenos Aires, Argentina.
Kronberg, María Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Kronberg, María Florencia. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Galvagno, Miguel Angel. Universidad Nacional de San Martín (UNSAM). Instituto de Investigaciones Biotecnológicas (IIBIO). San Martín, Buenos Aires, Argentina.
Galvagno, Miguel Angel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica (INMIBO). Ciudad Universitaria, Buenos Aires, Argentina.
Galvagno, Miguel Angel. CONICET - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica (INMIBO). Ciudad Universitaria, Buenos Aires, Argentina.
Año:
2021
Título revista:
Biocatalysis and Agricultural Biotecnology
ISSN:
1878-8181
Volumen:
36
Páginas:
e102116, 8 p.
Temas:
FEED INDUSTRY; NUTRITIONAL ADDITIVE; YEAST; PHYTASE; SCHWANNIOMYCES OCCIDENTALIS; SMALLANTHUS SONCHIFOLIUS (YACON)
Idioma:
Inglés
URL al Editor:

Resumen:

The suitability of the yeast Schwanniomyces (f. Debaryomyces) occidentalis to produce phytase activity on a Smallanthus sonchifolius (yacon) extract-based medium (YSM) was studied. Optimal conditions for phytase production were evaluated in YSM at 28 ◦C and pH 5.5, with an inoculum of 107 cells/mL, in shaken flasks, using a Box-Benkhen optimization design, and analyzed by response surface methodology. Phytase location was determined by subcellular fractioning and protoplast formation. Maximum phytase titres were obtained with 0.11% w/v yacon reducing sugar, 0.65% w/v (NH4)2SO4 and 16 h of fermentation. The maximum phytase activity attained under these conditions was over 6-fold higher than in non-optimized medium. Scaling-up in a STR-type bioreactor further increased phytase productivity by 1.5-fold. Phytase produced was associated to the cell wall fraction, and presented optimal temperature and pH range values of 75–80 ◦C and 4.0–5.0, respectively, retaining 80% of its activity at 80 ◦C for 40 min. Progressively increasing phosphorus concentration in the medium inhibited enzyme production, up to 60% with 0.1% w/v KH2PO4. Yeast biomass, phytase production and production kinetics in YSM were similar to those produced in a cane molasses-based medium. Yacon resulted in a promising alternative feed stock to produce a yeast-bound phytase potentially applicable in the feed industry as a nutritional additive.

Citación:

---------- APA ----------

Conde Molina, D.; Novelli Poisson, G. F.; Kronberg, M. F.; & Galvagno, M. A. (2021). Valorization of an Andean crop (yacon) through the production of a yeast cell - bound phytase. Biocatalysis and Agricultural Biotecnology,36,e102116, 8 p.
10.1016/j.bcab.2021.102116

---------- CHICAGO ----------

Conde Molina, Débora, Novelli Poisson, Guido Fernando, Kronberg, María Florencia, Galvagno, Miguel Angel. 2021. "Valorization of an Andean crop (yacon) through the production of a yeast cell - bound phytase". Biocatalysis and Agricultural Biotecnology 36:e102116, 8 p..
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