Facultad de Agronomia - UBA

Artículos FAUBA




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Acceso al documento : Documento de Acceso Restringido. Disponible sólo para la Comunidad FAUBA.
URL al registro : http://ri.agro.uba.ar/cgi-bin/library.cgi?a=d&c=arti&d=2012Cenzano
Tipo de material : Artículo
Autores : Cenzano, A. M.; Cantoro, R.; Hernández Sotomayor, S. M. T.; Abdala, G. I.; Racagni, G. E.
Título en inglés : Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
Fuente : Journal of Agricultural and Food Chemistry
Año : 2012
Volumen y número : Vol.60
Temas : ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY; GALACTOLIPID; KINASE; PHOSPHOLIPID; POTATO; SOLANUM TUBEROSUM; BIOCHEMISTRY; ENZYMES; FATTY ACIDS; GLYCEROL; IONIZATION; MASS SPECTROMETRY; METABOLISM; PHOSPHOLIPIDS; PHOSPHORYLATION; ELECTROSPRAY IONIZATION; PHOSPHOTRANSFERASE; VEGETABLE PROTEIN; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; ENZYMOLOGY; EVALUATION; GROWTH, DEVELOPMENT AND AGING; LIPID METABOLISM; METABOLISM; METHODOLOGY; PLANT TUBER; PHOSPHOTRANSFERASES; PLANT PROTEINS; PLANT TUBERS; SOLANUM TUBEROSUM; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION
Idioma : inglés
Páginas :  p.418-426
Resumen : There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation.
DOI : 10.1021/jf204269y
Link al editor : http://pubs.acs.org/
Cita recomendada : Cenzano, A. M., Cantoro, R., Hernández-Sotomayor, S. M. T., Abdala, G. I., & Racagni, G. E. (2012). Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons. Journal of Agricultural and Food Chemistry‚ 60‚ (1) p.418-426. Doi: 10.1021/jf204269y.
 
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