Facultad de Agronomia - UBA

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Acceso al documento : Documento de Acceso Restringido. Disponible sólo para la Comunidad FAUBA.
URL al registro : http://ri.agro.uba.ar/cgi-bin/library.cgi?a=d&c=arti&d=2010Demkura
Tipo de material : Artículo
Autores : Demkura, P.V.; Abdala, G.; Baldwin, I. T.; Ballaré, C. L.
Título en inglés : Jasmonate-dependent and -independent pathways mediate specific effects of solar ultraviolet B radiation on leaf phenolics and antiherbivore defense
Fuente : Plant Physiology
Año : 2010
Volumen y número : Vol.152
Temas : ACETIC ACID DERIVATIVE; CYCLOPENTANE DERIVATIVE; JASMONIC ACID; JASMONIC ACID METHYL ESTER; OXYLIPIN; PHENOL DERIVATIVE; GENE SILENCING; GENETICS; METABOLISM; PLANT LEAF; RADIATION EXPOSURE; SIGNAL TRANSDUCTION; SUNLIGHT; TOBACCO; TRANSGENIC PLANT; ULTRAVIOLET RADIATION; ACETIC ACIDS; ANIMALS; CYCLOPENTANES; INSECTS; OXYLIPINS; PHENOLS; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; ULTRAVIOLET RAYS; HEXAPODA; NICOTIANA ATTENUATA
Idioma : inglés
Páginas :  p.1084-1095
Resumen : Ultraviolet B (UV-B) radiation, a very small fraction of the daylight spectrum, elicits changes in plant secondary metabolism that have large effects on plant-insect interactions. The signal transduction pathways that mediate these specific effects of solar UV-B are not known. We examined the role of jasmonate signaling by measuring responses to UV-B in wild-type and transgenic jasmonate-deficient Nicotiana attenuata plants in which a lipoxygenase gene (NaLOX3) was silenced (as-lox). In wild-type plants, UV-B failed to elicit the accumulation of jasmonic acid (JA) or the bioactive JA-isoleucine conjugate but amplified the response of jasmonate-inducible genes, such as trypsin proteinase inhibitor (TPI), to wounding and methyl jasmonate, and increased the accumulation of several phenylpropanoid derivatives. Some of these phenolic responses (accumulation of caffeoyl-polyamine conjugates) were completely lacking in as-lox plants, whereas others (accumulation of rutin and chlorogenic acid) were similar in both genotypes. In open field conditions, as-lox plants received more insect damage than wild-type plants, as expected, but the dramatic increase in resistance to herbivory elicited by UV-B exposure, which was highly significant in wild-type plants, did not occur in as-lox plants. We conclude that solar UV-B (1) uses jasmonate-dependent and -independent pathways in the elicitation of phenolic compounds, and (2) increases sensitivity to jasmonates, leading to enhanced expression of wound-response genes (TPI). The lack of UV-B-induced antiherbivore protection in as-lox plants suggests that jasmonate signaling plays a central role in the mechanisms by which solar UV-B increases resistance to insect herbivores in the field.
DOI : 10.1104/pp.109.148999
Link al editor : http://my.aspb.org/
Cita recomendada : Demkura, P. V., Abdala, G., Baldwin, I. T., & Ballaré, C. L. (2010). Jasmonate-dependent and -independent pathways mediate specific effects of solar ultraviolet B radiation on leaf phenolics and antiherbivore defense. Plant Physiology‚ 152‚ (2) p.1084-1095. Doi: 10.1104/pp.109.148999.
 
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